The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
In mounting these objects for the microscope it is well to have the
part of the insect from which the scales are usually taken as a
separate slide, so that the natural arrangement of them may be seen.
This is easily accomplished with the wings of butterflies, gnats, &c.;
as they require no extraordinary care. In mounting the _scales_ they
may be placed upon slides, by passing the wings over the surface, or
by gently scraping the wing upon the slide, when they must be covered
with the thin glass. Of course, the extreme tenuity of these objects
does away with the necessity of any cell excepting that formed by the
gold-size or other cement used to attach the cover. The scales of the
podura should be placed upon the slide in a somewhat different manner.
This insect is without wings, and is no longer than the common flea. It
is often found amongst the sawdust in wine-cellars, continually leaping
about by the aid of its tail, which is bent underneath its body. Dr.
Carpenter says:--“Poduræ may be obtained by sprinkling a little oatmeal
on a piece of black paper near their haunts; and after leaving it there
for a few hours, removing it carefully to a large glazed basin, so
that, when they leap from the paper (as they will when brought to the
light), they may fall into the basin, and may thus separate themselves
from the meal. The best way of obtaining their scales, is to confine
several of them together beneath a wine glass inverted upon a piece of
fine smooth paper; for the scales will become detached by their leaps
against the glass, and will fall upon the paper.” These scales are
removed to the slide, and mounted as those from the gnats, &c. When the
podura has been caught without the aid of the meal, it may be placed
upon the slide, under a test-tube, or by any other mode of confinement,
and thus save the trouble of transfer from the paper before mentioned.
Another method is to seize the insect by the leg with the forceps and
drag it across the slide, when a sufficient quantity of scales will
probably be left upon it.
These scales are usually mounted “dry;” but Hogg recommends the use of
Canada balsam (Chapter III.) as rendering their structure more definite
when illuminated with Wenham’s parabolic reflector. Some advise other
methods, which will be mentioned in Chapter IV. As most _insects_
when undissected are mounted in Canada balsam, the different modes of
treatment which they require will be stated in another place.
Public-domain text, read in full here on John Shaqi.
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